Recombinant Cyclin E2 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87023A
Western blot detection of Cyclin E2 in Jurkat cell lysates using Cyclin E2 Rabbit mAb(1:500 diluted).Predicted band size:47kDa.Observed band size:47kDa.
Immunohistochemistry of Cyclin E2 in paraffin-embedded Human lung cancer tissue using Cyclin E2 Rabbit mAb at dilution 1:50
Immunofluorescence of Cyclin E2 (green) in MCF-7 using Cyclin E2 antibody at dilution 1:20, and DAPI(blue)
Product Name
Recombinant Cyclin E2 Monoclonal Antibody
Catalog Number
RD87023A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition.
Immunogen Information
Immunogen
A synthetic peptide of human Cyclin E2
Gene ID
9134
Swissprot
O96020
Synonyms
CCNE2CYCE2cyclin E2
Calculated MW
83kDa
Observed MW
83kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas