Recombinant Cytochrome C Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86922A
Western blot detection of Cytochrome C in Jurkat,Rat Brain,C6 cell lysates using Cytochrome C Rabbit mAb(1:1000 diluted).Predicted band size:12kDa.Observed band size:14kDa.
Immunohistochemistry of Cytochrome C in paraffin-embedded Human tonsil using Cytochrome C Rabbit mAb at dilution 1:100
Product Name
Recombinant Cytochrome C Monoclonal Antibody
Catalog Number
RD86922A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
Immunogen Information
Immunogen
A synthetic peptide of human Cytochrome C
Gene ID
54205
Swissprot
P99999
Synonyms
Cytochrome CSomaticCYCCytochrome CTHC4HCS
Calculated MW
47kDa
Observed MW
47kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.