Recombinant DGKA Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD87024A
Western blot detection of DGKA in Hela,A549 cell lysates using DGKA Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:83kDa.
Western blot detection of DGKA in Hela,A549 cell lysates using DGKA Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:83kDa.
Immunohistochemistry of DGKA in paraffin-embedded Human tonsil using DGKA Rabbit mAb at dilution 1:50
Immunohistochemistry of DGKA in paraffin-embedded Human tonsil using DGKA Rabbit mAb at dilution 1:50
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
Recombinant DGKA Monoclonal Antibody
Catalog Number
RD87024A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Immunogen Information

Circle
Immunogen
Recombinant protein of human DGKA
Gene ID
1606
Swissprot
P23743
Synonyms
80 kDa diacylglycerol kinaseDAG kinase alphaDAGKDAGK1DGK alphaDGK-alphadgkADGKADiacylglycerol kinase alphaDiacylglycerol kinasealpha 80kDaDiglyceride kinase alphaMGC12821MGC42356OTTHUMP00000242836OTTHUMP00000242955OTTHUMP00000244046
Calculated MW
41kDa
Observed MW
41kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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