Western blot detection of DGKZ/DGK-zeta in Hela,A549,HL-60,U2OS cell lysates using DGKZ/DGK-zeta Rabbit mAb(1:1000 diluted).Predicted band size:104kDa.Observed band size:124,114kDa.
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.
Immunogen Information
Immunogen
Recombinant protein of human DGKZ/DGK-zeta
Gene ID
8525
Swissprot
Q13574
Synonyms
DAG kinase zetaDAGK5DAGK5 PENDAGK6DGK ZETADGK-zetaDgkzDGKZDiacylglycerol kinase zetaDiglyceride kinase zetahDGKzeta
Calculated MW
104kDa
Observed MW
124/114kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.