Recombinant Dnmt3a Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86976A
Western blot detection of Dnmt3a in Hela,A549 cell lysates using Dnmt3a Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:130kDa.
Variation
Quantity
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Overview
Product Name
Recombinant Dnmt3a Monoclonal Antibody
Catalog Number
RD86976A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
A synthetic peptide of human Dnmt3a
Gene ID
1788
Swissprot
Q9Y6K1
Synonyms
DNA (cytosine 5) methyltransferase 3 alphaDNA (cytosine 5) methyltransferase 3ADNA (cytosine-5)-methyltransferase 3ADNA cytosine methyltransferase 3A2DNA methyltransferase 3 alphaDNA methyltransferase 3aDNA methyltransferase HsaIIIADNA MTase HsaIII
Calculated MW
119kDa
Observed MW
119kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas