Recombinant E2F1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87026A
Western blot detection of E2F1 in C6,3T3 cell lysates using E2F1 Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:70kDa.
Immunohistochemistry of E2F1 in paraffin-embedded Human tonsil using E2F1 Rabbit mAb at dilution 1:20
Immunofluorescence of E2F1(green) in Hela cells using E2F1 Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant E2F1 Monoclonal Antibody
Catalog Number
RD87026A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
Immunogen Information
Immunogen
A synthetic peptide of human E2F1
Gene ID
1869
Swissprot
Q01094
Synonyms
E2F-1RBAP1RBBP3RBP3E2F1
Calculated MW
69kDa
Observed MW
80kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:20-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas