Recombinant Eg5 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot detection of Eg5 in Hela,A549,HL-60,U2OS,C6 cell lysates using Eg5 Rabbit mAb(1:1000 diluted).Predicted band size:119kDa.Observed band size:119kDa.
Immunofluorescence of Eg5 (green) in hela using Eg5 Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant Eg5 Monoclonal Antibody
Catalog Number
RD86977A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
Immunogen Information
Immunogen
Recombinant protein of human Eg5
Gene ID
3832
Swissprot
P52732
Synonyms
KIF11EG5HKSPKNSL1MCLMRTRIP5
Calculated MW
70kDa
Observed MW
60/64kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas