Recombinant EGFR Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86912A
Western blot detection of EGFR in Hela cell lysates using EGFR Rabbit mAb(1:1000 diluted).Predicted band size:134kDa.Observed band size:175kDa.
Immunofluorescence of EGFR(green) in Hela cells using EGFR Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant EGFR Monoclonal Antibody
Catalog Number
RD86912A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
EGFR,also named as ERBB1,is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12,encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression,including cell proliferation,apoptosis,angiogenesis,and metastatic spread. Mutations in this gene are associated with lung cancer.
Immunogen Information
Immunogen
A synthetic peptide of human EGFR
Gene ID
1956
Swissprot
P00533
Synonyms
Cell growth inhibiting protein 40Cell proliferation inducing protein 61EGF REGFREpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)Epidermal growth factor receptorerb-b2 receptor tyrosine kinase 1ERBBE
Calculated MW
88kDa
Observed MW
88kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas