Recombinant EpCAM Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87028A
Western blot detection of EpCAM in MCF7,HCT116,T47D,Hela cell lysates using EpCAM Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:40kDa.
Immunohistochemistry of EpCAM in paraffin-embedded Human breast cancer tissue using EpCAM Rabbit mAb at dilution 1:50
Immunofluorescence of EpCAM (green) in MCF-7 using EpCAM antibody at dilution 1:20, and DAPI(blue)
Product Name
Recombinant EpCAM Monoclonal Antibody
Catalog Number
RD87028A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Epithelial cell adhesion molecule (EpCAM,CD326) is a type I transmembrane glycoprotein that functions as a homophilic,epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion,EpCAM is also involved in cellular signaling,cell migration,proliferation,and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas,and has become a therapeutic target.
Immunogen Information
Immunogen
Recombinant protein of human EpCAM
Gene ID
4072
Swissprot
P16422
Synonyms
17 1A323/A3Adenocarcinoma associated antigenAdenocarcinoma-associated antigenAntigen identified by monoclonal AUA1AUA1CD326CD326 antigenCell surface glycoprotein Trop 1Cell surface glycoprotein Trop 2Cell surface glycoprotein Trop-1CO 17ACO17
Calculated MW
38kDa
Observed MW
45kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas