Recombinant ERK1/2 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86905A
Western blot detection of p42 MAPK (ERK2) in K562,Rat Brain,C6,3T3,Hela cell lysates using p42 MAPK (ERK2) Rabbit mAb(1:1000 diluted).Predicted band size:43,41 kDa.Observed band size:44,42kDa.
Western blot detection of p42 MAPK (ERK2) in K562,Rat Brain,C6,3T3,Hela cell lysates using p42 MAPK (ERK2) Rabbit mAb(1:1000 diluted).Predicted band size:43,41 kDa.Observed band size:44,42kDa.
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Overview

Circle
Product Name
Recombinant ERK1/2 Monoclonal Antibody
Catalog Number
RD86905A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).

Immunogen Information

Circle
Immunogen
Recombinant protein of human ERK2
Gene ID
5595/5594
Swissprot
P27361/P28482
Synonyms
ERKp38p40p41ERK2ERT1ERK-2MAPK2PRKM1PRKM2P42MAPKp41mapkp42-MAPKERK1ERT2ERK-1PRKM3P44ERK1P44MAPKHS44KDAPHUMKER1Ap44-ERK1p44-MAPK
Calculated MW
21kDa
Observed MW
20kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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