Recombinant Glucocorticoid Receptor Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot detection of Glucocorticoid Receptor in K562,3T3,Hela cell lysates using Glucocorticoid Receptor Rabbit mAb(1:1000 diluted).Predicted band size:86kDa.Observed band size: 94,91kDa.
Immunofluorescence of Glucocorticoid Receptor(green) in Hela cells using Glucocorticoid Receptor Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant Glucocorticoid Receptor Monoclonal Antibody
Catalog Number
RD87034A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:10678832). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay . Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (PubMed:15037546).
Immunogen Information
Immunogen
Recombinant protein of mouse Glucocorticoid Receptor
Gene ID
2908
Swissprot
P06537
Synonyms
GRGCRGRLGCCRGCRST
Calculated MW
56kDa
Observed MW
51kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.