For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86939A
Western blot detection of DFNA5/GSDME in U251MG,U2OS,HepG2,Hela cell lysates using DFNA5/GSDME Rabbit mAb(1:500 diluted).Predicted band size:55kDa.Observed band size:55kDa.
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant protein of human DFNA5/GSDME
Gene ID
1687
Swissprot
O60443
Synonyms
2310037D07Rik4932441K13RikDeafnessautosomal dominant 5Deafnessautosomal dominant 5 proteinDFNA5DFNA5 geneDFNA5Dfna5hEG14210Fin15ICERE 1ICERE-1Inversely correlated with estrogen receptor expression 1Non-syndromic hearing impairment protein 5
Calculated MW
66kDa
Observed MW
66kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.