Recombinant GTPase HRAS Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87037A
Western blot detection of Ras in Hela,CHO-K1,C6 cell lysates using Ras Rabbit mAb(1:1000 diluted).Predicted band size:21kDa.Observed band size:21kDa.
Product Name
Recombinant GTPase HRAS Monoclonal Antibody
Catalog Number
RD87037A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein.
Immunogen Information
Immunogen
A synthetic peptide of human Ras
Gene ID
3265 ,3845 ,4893
Swissprot
P01111
Synonyms
ALPS4AV095280GTPase NRasHRAS1N rasN ras protein part 4Neuroblastoma RAS viral (v ras) oncogene homologNRASNRAS1NS6OTTHUMP00000013879OTTMUSP00000023521RASNTransforming protein N RasTransforming protein N-Rasv ras neuroblastoma RAS viral onco
Calculated MW
87kDa
Observed MW
87kDa
Applications
Reactivity
Human,Mouse,Rat,Hamster
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas