For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86915A
Western blot detection of Heme Oxygenase 1 in 3T3 cell lysates using Heme Oxygenase 1 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:33kDa.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Immunogen Information
Immunogen
A synthetic peptide of human Heme Oxygenase 1
Gene ID
3162
Swissprot
P09601
Synonyms
32 kDbK286B10D8Wsu38eheat shock protein 32 kDheat shock protein 32kDHeat shock proteinHeme oxygenase (decycling) 1Heme oxygenase 1HemoxHMOX 1HmoxHmox1HMOX1HO 1HOHO-1HO1Hsp32
Calculated MW
49kDa
Observed MW
57-65kDa
Applications
Reactivity
Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.