Recombinant Hexokinase II Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86947A
Western blot detection of Hexokinase II in Hela,CHO-K1 cell lysates using Hexokinase II Rabbit mAb(1:500 diluted).Predicted band size:102kDa.Observed band size:102kDa.
Immunohistochemistry of Hexokinase II in paraffin-embedded Human breast cancer tissue using Hexokinase II Rabbit mAb at dilution 1/50
Product Name
Recombinant Hexokinase II Monoclonal Antibody
Catalog Number
RD86947A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Immunogen Information
Immunogen
Recombinant protein of human Hexokinase II
Gene ID
3099
Swissprot
P52789
Synonyms
DKFZp686M1669Hexokinase 2Hexokinase 2 muscleHexokinase type IIHexokinase-2HK 2HK IIHK2HKIIHxK 2HxK2HXK2Muscle form hexokinase
Calculated MW
95kDa
Observed MW
95kDa
Applications
Reactivity
Human,Rat,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas