Recombinant Hsp70 1A Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87040A
Western blot detection of Hsp70 in K562,Rat Brain,C6,3T3 cell lysates using Hsp70 Rabbit mAb(1:1000 diluted).Predicted band size:70kDa.Observed band size:70kDa.
Immunohistochemistry of Hsp7 in paraffin-embedded Human tonsil using Hsp7 Rabbit mAb at dilution 1:50
Immunofluorescence of Hsp70(green) in Hela cells using Hsp70 Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant Hsp70 1A Monoclonal Antibody
Catalog Number
RD87040A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Immunogen Information
Immunogen
A synthetic peptide of human Hsp70
Gene ID
3303
Swissprot
P0DMV8
Synonyms
heat shock protein family A (Hsp70) member 1Aheat shock protein family A (Hsp70) member 1BHSP70-1BHSP70-2HSP70.2
Calculated MW
85/83kDa
Observed MW
90kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas