Recombinant ILF3 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86948A
Western blot detection of ILF3/NF90 in Jurkat,C6,Hela cell lysates using ILF3/NF90 Rabbit mAb(1:1000 diluted).Predicted band size:95kDa.Observed band size:95kDa.
Immunohistochemistry of ILF3 in paraffin-embedded Human lung cancer tissue using ILF3 Rabbit mAb at dilution 1:50
Immunofluorescence of ILF3(green) in hela using ILF3 Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant ILF3 Monoclonal Antibody
Catalog Number
RD86948A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen Information
Immunogen
A synthetic peptide of human ILF3
Gene ID
3609
Swissprot
Q12906
Synonyms
CBTFDouble stranded RNA binding protein 76Double-stranded RNA-binding protein 76DRBFDRBP76ILF3ILF3Interleukin enhancer binding factor 3Interleukin enhancer-binding factor 3M phase phosphoprotein 4M-phase phosphoprotein 4MPHOSPH4MPP4NF AT 90N
Calculated MW
131kDa
Observed MW
131kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas