For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87046A
Western blot detection of Integrin alpha 5 in K562,3T3 cell lysates using Integrin alpha 5 Rabbit mAb(1:1000 diluted).Predicted band size:115kDa.Observed band size:150kDa.
Immunohistochemistry of Integrin alpha 5 in paraffin-embedded Human breast cancer tissue using Integrin alpha 5 Rabbit mAb at dilution 1:50
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes.
Immunogen Information
Immunogen
A synthetic peptide of human Integrin alpha 5
Gene ID
3678
Swissprot
P08648
Synonyms
CD49 antigen-like family member ECD49eFibronectin receptor subunit alphaFibronectin receptoralpha subunitFNRAIntegrin alpha 5 (fibronectin receptor alpha)Integrin alpha-5Integrin alpha-5 light chainIntegrin alpha-FIntegrinalpha 5 (fibronectin r
Calculated MW
88kDa
Observed MW
88kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.