Recombinant JNK Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86950A
Western blot detection of JNK1/2/3 in C6,3T3 cell lysates using JNK1/2/3 Rabbit mAb(1:1000 diluted).Predicted band size:48,53kDa.Observed band size: 46,54kDa.
Immunofluorescence of JNK1/2/3 (green) in Hela cells using JNK1/2/3 Rabbit mAb at dilution 1:100, and DAPI(blue)
Product Name
Recombinant JNK Monoclonal Antibody
Catalog Number
RD86950A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1,PRKM8,SAPK1,SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73,mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.
Immunogen Information
Immunogen
Recombinant protein of human JNK1
Gene ID
5599
Swissprot
P45983
Synonyms
AI849689c Jun N terminal kinase 1C-JUN kinase 1c-Jun N-terminal kinase 1EC 2.7.11.24JNK 1JNKJNK-46JNK1A2JNK21B1/2MAP kinase 8MAPK 8mapk8Mitogen activated protein kinase 8Mitogen-activated protein kinase 8MK08p54 gammaPrkm8Protein kinase
Calculated MW
69kDa
Observed MW
69kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas