Recombinant Keap1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86978A
Western blot detection of Keap1 in Hela,A549,C2C12 cell lysates using Keap1 Rabbit mAb(1:1000 diluted).Predicted band size:70kDa.Observed band size:60-64kDa.
Immunohistochemical of Keap1 in Human breast cancer tissue using Keap1 antibody at dilution 1:20
Product Name
Recombinant Keap1 Monoclonal Antibody
Catalog Number
RD86978A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
KEAP1,also named as INRF2,KIAA0132 and KLHL19,is part of a multiprotein complex that contains the CUL3-ROC1 ubiquitin ligase,which can ubiquitinate the N-terminal domain of NRF2. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2,the
ETGE and DLG sites,which affect the KEAP1-NRF2 interaction and/or its physiological consequences. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It also retains BPTF in the cytosol. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human KEAP1.
Immunogen Information
Immunogen
Recombinant protein of human Keap1
Gene ID
9817
Swissprot
Q14145
Synonyms
Cytosolic inhibitor of Nrf2INrf 2INrf2Keap 1KEAP1KEAP1Kelch like ECH associated protein 1Kelch like family member 19Kelch like protein 19Kelch-like ECH-associated protein 1Kelch-like protein 19KIAA0132KLHL 19KLHL19MGC10630MGC1114MGC20887M
Calculated MW
40kDa
Observed MW
42kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:20-1:50
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas