Recombinant LC3A Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87051A
Western blot detection of LC3A in A549,MCF-7,293T,C2C12 cell lysates using LC3A Rabbit mAb(1:1000 diluted).Predicted band size:14kDa.Observed band size:16kDa.
Product Name
Recombinant LC3A Monoclonal Antibody
Catalog Number
RD87051A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
LC3,also known as microtubule-associated protein 1 light chain 3 (MAP1LC3),is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B,and LC3C. LC3 is first synthesized as pro-LC3,and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
Immunogen Information
Immunogen
A synthetic peptide of human MAP1LC3A
Gene ID
84557
Swissprot
Q9H492
Synonyms
ATG8EAutophagy-related protein LC3 AAutophagy-related ubiquitin-like modifier LC3 ALC3LC3AMAP1 light chain 3 like protein 1MAP1 light chain 3-like protein 1MAP1A/1B light chain 3 AMAP1A/MAP1B LC3 AMAP1A/MAP1B light chain 3 AMAP1ALC3MAP1BLC3Map
Calculated MW
85kDa
Observed MW
85kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas