For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87054A
Western blot detection of MCM2 in K562,C6,3T3,Hela cell lysates using MCM2 Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.
Immunofluorescence of MCM2(green) in Hela cells using MCM2 Rabbit mAb at dilution 1:50, and DAPI(blue)
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with mCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Immunogen Information
Immunogen
A synthetic peptide of human MCM2
Gene ID
4171
Swissprot
P49736
Synonyms
BM28CCNL 1CCNL1CDC like 1cdc19CDCL 1CDCL1Cell devision cycle like 1Cyclin like 1D3S3194DNA replication licensing factor MCM2KIAA0030MCM 2MCM2MCM2 minichromosome maintenance deficient 2 mitotinMCM2 minichromosome maintenance deficient 2 mito
Calculated MW
110kDa
Observed MW
210kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.