Recombinant MCM5 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86902A
Western blot detection of MCM5 in Jurkat,C6,Hela cell lysates using MCM5 Rabbit mAb(1:1000 diluted).Predicted band size:82kDa.Observed band size:82kDa.
Western blot detection of MCM5 in Jurkat,C6,Hela cell lysates using MCM5 Rabbit mAb(1:1000 diluted).Predicted band size:82kDa.Observed band size:82kDa.
Immunohistochemistry of MCM5 in paraffin-embedded Human tonsil using MCM5 Rabbit mAb at dilution 1:100
Immunohistochemistry of MCM5 in paraffin-embedded Human tonsil using MCM5 Rabbit mAb at dilution 1:100
Immunofluorescence of MCM5(green) in Hela cells using MCM5 Rabbit mAb at dilution 1:200, and DAPI(blue)
Immunofluorescence of MCM5(green) in Hela cells using MCM5 Rabbit mAb at dilution 1:200, and DAPI(blue)
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
Recombinant MCM5 Monoclonal Antibody
Catalog Number
RD86902A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Immunogen Information

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Immunogen
A synthetic peptide of human MCM5
Gene ID
4174
Swissprot
P33992
Synonyms
CDC 46CDC46CDC46 homologCell division cycle 46DNA replication licensing factorMCM 5MGC5315Minichromosome maintenance complex component 5P1 CDC46
Calculated MW
36kDa
Observed MW
39kDa

Applications

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Reactivity
Human,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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