Recombinant MHC Class I Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86992A
Western blot detection of MHC class I in Hela,A549 cell lysates using MHC class I Rabbit mAb(1:1000 diluted).Observed band size:41kDa.
Western blot detection of MHC class I in Hela,A549 cell lysates using MHC class I Rabbit mAb(1:1000 diluted).Observed band size:41kDa.
Immunohistochemical of MHC class I in Human tonsil tissue using MHC class I antibody at dilution 1:50
Immunohistochemical of MHC class I in Human tonsil tissue using MHC class I antibody at dilution 1:50
Variation
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Overview
Circle
Product Name
Recombinant MHC Class I Monoclonal Antibody
Catalog Number
RD86992A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Circle
Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. HLA-A, -B and -C encode membrane anchored heavy chains which heterodimerize with a light chain (b-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-A, -B and -C alleles.
Immunogen Information
Circle
Immunogen
A synthesized peptide derived from human MHC class I
Swissprot
P30450
Synonyms
HLA class I histocompatibilityHLA AHLA B
Calculated MW
13kDa
Observed MW
15/20kDa
Applications
Circle
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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