Recombinant MLH1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot detection of MLH1 in K562,C6,3T3,Hela cell lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.
Immunofluorescence of MLH1(green) in Hela cells using MLH1 Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant MLH1 Monoclonal Antibody
Catalog Number
RD87056A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
Immunogen Information
Immunogen
A synthetic peptide of human MLH1
Gene ID
4292
Swissprot
P40692
Synonyms
COCA 2COCA2DNA mismatch repair protein Mlh1FCC 2FCC2hMLH 1hMLH1HNPCC 2HNPCCHNPCC2MGC5172MLH 1MLH1MLH1MutL homolog 1 (E. coli)MutL homolog 1MutL homolog 1 colon cancer nonpolyposis type 2MutL homolog 1colon cancernonpolyposis type 2 (E.
Calculated MW
29kDa
Observed MW
24kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas