Recombinant mtTFA Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot detection of mtTFA in K562,Hela cell lysates using mtTFA Rabbit mAb(1:1000 diluted).Predicted band size:29kDa.Observed band size:24kDa.
Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using mtTFA Rabbit mAb at dilution 1:100
Immunofluorescence of mtTFA/TFAM(green) in Hela cells using mtTFA/TFAM Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant mtTFA Monoclonal Antibody
Catalog Number
RD87057A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.
TFAM (Transcription Factor A, Mitochondrial) is a Protein Coding gene. Diseases associated with TFAM include Mitochondrial Dna Depletion Syndrome 15, Hepatocerebral Type and Skin Carcinoma In Situ. Among its related pathways are Mitochondrial Gene Expression and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. GO annotations related to this gene include poly(A) RNA binding and chromatin binding.
Immunogen Information
Immunogen
A synthetic peptide of human mtTFA
Gene ID
7019
Swissprot
Q00059
Synonyms
anscription factor 6-like 1Mitochondrial transcription factor 1mitochondrial transcription factor AMtTF1mtTFATCF 6TCF-6TCF6TCF6L1TCF6L2TCF6L3TFAMTFAMTranscription factor 6Transcription factor 6 like 2 (mitochondrial transcription factor)Tra
Calculated MW
149kDa
Observed MW
115kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.