Western blot detection of NEDD4 in Hela,A549,HL-60,U2OS,U87-MG cell lysates using NEDD4 Rabbit mAb(1:1000 diluted).Predicted band size:149kDa.Observed band size:115kDa.
This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
Immunogen Information
Immunogen
A synthetic peptide of human NEDD4
Gene ID
4734
Swissprot
P46934
Synonyms
NEDD4NEDD4-1RPF1
Calculated MW
35kDa
Observed MW
35kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:3000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.