For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87060A
Western blot detection of NR1D1 in K562,C6,3T3,Hela cell lysates using NR1D1 Rabbit mAb(1:1000 diluted).Predicted band size:67kDa.Observed band size:67kDa.
Immunofluorescence of NR1D1(green) in Hela cells using NR1D1 Rabbit mAb at dilution 1:50, and DAPI(blue)
This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes.
Immunogen Information
Immunogen
A synthetic peptide of human NR1D1
Gene ID
9572
Swissprot
P20393
Synonyms
EAR-1EAR1ERBA-related 1hRevNr1d1NR1D1Nuclear receptor Rev ErbA alphaNuclear receptor subfamily 1 group D member 1Rev erbAalphaRev erbalphaRev-erbA-alphaRev-ErbAalphaReverbaTHRA1THRALThyroid hormone receptoralpha likeThyroid hormone recept
Calculated MW
54kDa
Observed MW
68kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.