Recombinant Osteopontin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86969A
Western blot detection of Osteopontin in Hela,CHO-K1,C6,Jurkat cell lysates using Osteopontin Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:35kDa.
Immunohistochemistry of Osteopontin in paraffin-embedded Human colon cancer tissue using Osteopontin Rabbit mAb at dilution 1:50
Product Name
Recombinant Osteopontin Monoclonal Antibody
Catalog Number
RD86969A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human Osteopontin
Gene ID
6696
Swissprot
P10451
Synonyms
BNSPBSPIETA-1OPNOsteopontinSPP1
Calculated MW
15kDa
Observed MW
15kDa
Applications
Reactivity
Human,Rat,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas