Western blot detection of P Glycoprotein in Hela cell lysates using P Glycoprotein Rabbit mAb(1:1000 diluted).Predicted band size:142kDa.Observed band size:130-180kDa.
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance.
Immunogen Information
Immunogen
Recombinant protein of human P Glycoprotein
Gene ID
5243
Swissprot
P08183
Synonyms
ABC20ABCB1ATP binding cassettesub family B (MDR/TAP)member 1ATP-binding cassette sub-family B member 1CD243CLCSColchicin sensitivityDoxorubicin resistanceGP170MDR1MDR1Multidrug resistance 1Multidrug resistance protein 1P glycoprotein 1P gp
Calculated MW
16kDa
Observed MW
16kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.