Recombinant p53 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87065A
Western blot detection of p53 in Jurkat cell lysates using p53 Rabbit mAb(1:500 diluted).Predicted band size:44kDa.Observed band size:53kDa.
Immunofluorescence of p53 (green) in MCF-7 using p53 antibody at dilution 1:20, and DAPI(blue)
Product Name
Recombinant p53 Monoclonal Antibody
Catalog Number
RD87065A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
TP53,also named as P53 and NY-CO-13,belongs to the p53 family. It has 9 isoforms. In SDS-Page,the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
Immunogen Information
Immunogen
Recombinant protein of human p53
Gene ID
7157
Swissprot
P04637
Synonyms
Antigen NY-CO-13BCC7Cellular tumor antigen p53FLJ92943LFS1Mutant tumor protein 53p53p53 tumor suppressorP53Phosphoprotein p53Tp53Transformation related protein 53TRP53Tumor protein 53Tumor protein p53Tumor suppressor p53
Calculated MW
65kDa
Observed MW
65kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas