For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87066A
Western blot detection of Paxillin in CHO-K1,C6 cell lysates using Paxillin Rabbit mAb(1:1000 diluted).Predicted band size:65kDa.Observed band size:65kDa.
Immunohistochemical of Paxillin in Human breast cancer tissue using Paxillin antibody at dilution 1:20
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Immunogen Information
Immunogen
A synthetic peptide of human Paxillin
Gene ID
5829
Swissprot
P49023
Synonyms
PaxillinPXNpaxillin
Calculated MW
39kDa
Observed MW
35/45kDa
Applications
Reactivity
Human,Rat,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.