Recombinant PERK Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86955A
Western blot detection of PERK in K562,C6,3T3,Hela cell lysates using PERK Rabbit mAb(1:1000 diluted).Predicted band size:125kDa.Observed band size:140kDa.
Product Name
Recombinant PERK Monoclonal Antibody
Catalog Number
RD86955A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Immunogen Information
Immunogen
Recombinant protein of human PERK
Gene ID
9451
Swissprot
Q9NZJ5
Synonyms
DKFZp781H1925E2AK3EC 2.7.11.1Eif2ak3Eukaryotic translation initiation factor 2 alpha kinase 3Eukaryotic translation initiation factor 2-alpha kinase 3Heme regulated EIF2 alpha kinaseHRIHsPEKPancreatic eIF2 alpha kinasePancreatic eIF2-alpha kinas
Calculated MW
47kDa
Observed MW
54kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas