Recombinant PI3 Kinase p85 alpha Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86908A
Western blot detection of PI3 Kinase p85 alpha in Jurkat,C6,CHO-K1 cell lysates using PI3 Kinase p85 alpha Rabbit mAb(1:1000 diluted).Predicted band size:84kDa.Observed band size:84kDa.
Immunofluorescence of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody at dilution 1:20, and DAPI(blue)
Variation
Quantity
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Overview
Product Name
Recombinant PI3 Kinase p85 alpha Monoclonal Antibody
Catalog Number
RD86908A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of insulin and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer,which is composed of a P110 catalytic subunit and a P85 regulatory subunit,is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.
Immunogen Information
Immunogen
A synthetic peptide of human PI 3 Kinase p85 alpha
Gene ID
5295
Swissprot
P27986
Synonyms
GRB1p85 alphap85P85APhosphatidylinositol 3 kinase associated p 85 alphaPhosphatidylinositol 3 kinase regulatory 1Phosphatidylinositol 3 kinaseregulatory subunitpolypeptide 1 (p85 alpha)Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
Calculated MW
25kDa
Observed MW
25kDa
Applications
Reactivity
Human,Rat,Hamster
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas