Recombinant PKR Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot detection of PKR in C6,3T3 cell lysates using PKR Rabbit mAb(1:1000 diluted).Predicted band size:62kDa.Observed band size:74kDa.
Western blot detection of PKR in C6,3T3 cell lysates using PKR Rabbit mAb(1:1000 diluted).Predicted band size:62kDa.Observed band size:74kDa.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
Recombinant PKR Monoclonal Antibody
Catalog Number
RD87070A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Immunogen Information

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Immunogen
Recombinant protein of mouse PKR
Gene ID
19106
Swissprot
Q03963
Synonyms
EIF2AK2EIF2AK1PKRPPP1R83PRKREif2ak2
Calculated MW
37kDa
Observed MW
37kDa

Applications

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Reactivity
Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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