Western blot detection of PRMT7 in K562,3T3,Hela cell lysates using PRMT7 Rabbit mAb(1:1000 diluted).Predicted band size:79kDa.Observed band size:79kDa.
Protein arginine methyltransferase 7is aproteinthat in humans is encoded by thePRMT7gene.Argininemethylationis an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, usingS-adenosylmethionine(AdoMet) as the methyl donor. Arginine methylation is implicated insignal transduction, RNA transport, andRNA splicing. May be involved in etoposide-induced cytotoxicity, a chemotherapeutic agent frequently used for testicular cancer and small-cell lung cancer that can cause cytotoxicity in the treatment of other cancers. Down-regulation confers increased sensitivity to the Top1 inhibitor camptothecin (CPT).
Immunogen Information
Immunogen
A synthetic peptide of human PRMT7
Gene ID
54496
Swissprot
Q9NVM4
Synonyms
[Myelin basic protein]-arginine N-methyltransferase PRMT7ANM7FLJ10640Histone-arginine N-methyltransferase PRMT7KIAA1933Myelin basic protein arginine N methyltransferaseOTTHUMP00000174863PRMT7Protein arginine methyltransferase 7Protein arginine N-
Calculated MW
30 kDa
Observed MW
30 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.