Recombinant Prohibitin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87074A
Western blot detection of Prohibitin in Hela,A549,HL-60,U2OS,C6 using Prohibitin Rabbit mAb(1:1000 diluted)
Immunohistochemistry of Prohibitin in paraffin-embedded Human Cholangiocarcinoma using Prohibitin Rabbit mAb at dilution 1:50
Product Name
Recombinant Prohibitin Monoclonal Antibody
Catalog Number
RD87074A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Prohibitin,also known as PHB,a member of the Band-7 family of proteins,is widely distributed in bacteria,plants,yeast,protozoa and mammals and is important in cell proliferation,differentiation and apoptosis . This protein localizes to the inner membrane of mitochondria,where it acts as a chaperone protein,and is also found in the nucleus,where it negatively regulates transcription . Recent study confirmed that the expression and distribution of PHB,which is a nuclear matrix protein,affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis .
Immunogen Information
Immunogen
A synthetic peptide of human Prohibitin
Gene ID
5245
Swissprot
P35232
Synonyms
Epididymis luminal protein 215Epididymis secretory sperm binding protein Li 54eHEL 215HEL S 54ePHBPHBPHB1Prohibitin
Calculated MW
57 kDa
Observed MW
57 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.