Recombinant PTEN Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86956A
Western blot detection of PTEN in K562,C6,3T3,Hela cell lysates using PTEN Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:54kDa.
Product Name
Recombinant PTEN Monoclonal Antibody
Catalog Number
RD86956A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
PTEN (also designated MMAC1),products of tumor suppressor genes,are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors,such as brain,breast,kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.
Immunogen Information
Immunogen
A synthetic peptide of human PTEN
Gene ID
5728
Swissprot
P60484
Synonyms
10q23delBZSDECGLM2MGC11227MHAMMMAC1MMAC1 phosphatase and tensin homolog deleted on chromosome 10Mutated in multiple advanced cancers 1Phosphatase and tensin homologPhosphatase and tensin like proteinPhosphatidylinositol 345-trisphosphate 3-ph
Calculated MW
40kDa
Observed MW
40kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.