Recombinant Rad21 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86981A
Western blot detection of Rad21 in Hela,CHO-K1,Ramos using Rad21 Rabbit mAb(1:1000 diluted)
Immunohistochemistry of Rad21 in paraffin-embedded Human lung cancer tissue using Rad21 Rabbit mAb at dilution 1:50
Product Name
Recombinant Rad21 Monoclonal Antibody
Catalog Number
RD86981A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells
Immunogen Information
Immunogen
A synthetic peptide of human Rad21
Gene ID
5885
Swissprot
O60216
Synonyms
MGSHR21MCD1NXP1SCC1CDLS4hHR21HRAD21
Calculated MW
13kDa
Observed MW
13kDa
Applications
Reactivity
Human,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.