Recombinant RENT1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87076A
Western blot detection of RENT1 / hUPF1 in Jurkat,Hela cell lysates using RENT1 / hUPF1 Rabbit mAb(1:1000 diluted).Predicted band size:124kDa.Observed band size:124kDa.
Immunohistochemistry of RENT1 in paraffin-embedded Human Cholangiocarcinoma using RENT1 Rabbit mAb at dilution 1:50
Immunofluorescence of RENT1(green) in Hela using RENT1 Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant RENT1 Monoclonal Antibody
Catalog Number
RD87076A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human RENT1
Gene ID
5976
Swissprot
Q92900
Synonyms
ATP dependent helicase RENT1ATP-dependent helicase RENT1Delta helicaseFLJ43809FLJ46894HUPF 1hUpf1KIAA0221Nonsense mRNA reducing factor 1NORF 1NORF1pNORF 1pNORF1Regulator of nonsense transcripts 1RENT 1RENT1RENT1Smg 2Smg 2 homolog nonsens
Calculated MW
84 kDa
Observed MW
84 kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:2000-1:3000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas