For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86957A
Western blot detection of RKIP in Rat Brain,C6,3T3,Hela cell lysates using RKIP Rabbit mAb(1:1000 diluted).Predicted band size:40kDa.Observed band size:40kDa.
Immunohistochemistry of RKIP in paraffin-embedded Human tonsil using RKIP Rabbit mAb at dilution 1:50
Raf kinase inhibitor protein (RKIP),also termed phosphatidylethanolamine binding protein PEBP1,was initially identified as a potent inhibitor of Raf-1/MEK/ERK,NF-kB,and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.
Immunogen Information
Immunogen
A synthetic peptide of human RKIP
Gene ID
5037
Swissprot
P30086
Synonyms
Epididymis luminal protein 210Epididymis secretory protein Li 34Epididymis secretory protein Li 96HCNPHCNPppHEL 210HEL S 34HEL S 96Hippocampal cholinergic neurostimulating peptideNeuropolypeptide h3PBPPEBPPEBP 1PEBP-1Pebp1PEBP1Phosphatidyl
Calculated MW
96kDa
Observed MW
120kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.