Western blot detection of RKIP in Rat Brain,C6,3T3,Hela cell lysates using RKIP Rabbit mAb(1:1000 diluted).Predicted band size:40kDa.Observed band size:40kDa.
Immunohistochemistry of RKIP in paraffin-embedded Human tonsil using RKIP Rabbit mAb at dilution 1:50
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Overview
Product Name
Recombinant RKIP Monoclonal Antibody
Catalog Number
RD86957A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Raf kinase inhibitor protein (RKIP),also termed phosphatidylethanolamine binding protein PEBP1,was initially identified as a potent inhibitor of Raf-1/MEK/ERK,NF-kB,and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.
Immunogen Information
Immunogen
A synthetic peptide of human RKIP
Gene ID
5037
Swissprot
P30086
Synonyms
Epididymis luminal protein 210Epididymis secretory protein Li 34Epididymis secretory protein Li 96HCNPHCNPppHEL 210HEL S 34HEL S 96Hippocampal cholinergic neurostimulating peptideNeuropolypeptide h3PBPPEBPPEBP 1PEBP-1Pebp1PEBP1Phosphatidyl
Calculated MW
96kDa
Observed MW
120kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.