Recombinant S100A4 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87079A
Western blot detection of S100A4 in Hela cell lysates using S100A4 Rabbit mAb(1:500 diluted).Predicted band size:12kDa.Observed band size:12kDa.
Immunohistochemistry of S100A4 in paraffin-embedded Human lung cancer tissue using S100A4 Rabbit mAb at dilution 1:50
Immunofluorescence of S1A4(green) in hela using S1A4 Rabbit mAb at dilution 1/5, and DAPI(blue)
Product Name
Recombinant S100A4 Monoclonal Antibody
Catalog Number
RD87079A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo. It is an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis. Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.
Immunogen Information
Immunogen
A synthetic peptide of human S100A4
Gene ID
6275
Swissprot
P26447
Synonyms
18A242Acalcium Placental proteinCalvasculinCAPLFibroblast specific protein 1 (FSP1)Fibroblast specific protein 1Fibroblast specific proteinFSP1Leukemia multidrug resistance associated proteinMalignant transformation suppression 1 (MTS1)Malignan
Calculated MW
56kDa
Observed MW
70kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas