Recombinant Semaphorin 4D Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86958A
Western blot detection of Semaphorin 4D/CD100 in Jurkat cell lysates using Semaphorin 4D/CD100 Rabbit mAb(1:500 diluted).Predicted band size:96kDa.Observed band size:120kDa.
Product Name
Recombinant Semaphorin 4D Monoclonal Antibody
Catalog Number
RD86958A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
Immunogen Information
Immunogen
A synthetic peptide of human Semaphorin 4D
Gene ID
10507
Swissprot
Q92854
Synonyms
A8BB 18BB18CD 100CD100CD100 antigenColl 4COLL4Collapsin 4Collapsin4GR3Leukocyte activation antigen CD100M sema GMSEMASEM4DSema 4dSema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4DSema H
Calculated MW
52kDa
Observed MW
52kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas