Recombinant SF3B1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86983A
Western blot detection of SF3B1 in Hela,CHO-K1,C6,Ramos using SF3B1 Rabbit mAb(1:1000 diluted)
Immunohistochemistry of SF3B1 in paraffin-embedded Human lung cancer tissue using SF3B1 Rabbit mAb at dilution 1:50
Product Name
Recombinant SF3B1 Monoclonal Antibody
Catalog Number
RD86983A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
A synthetic peptide of human SF3B1
Gene ID
23451
Swissprot
O75533
Synonyms
SF3B1Hsh155MDSPRP10PRPF10SAP155SF3b155
Calculated MW
181kDa
Observed MW
190kDa
Applications
Reactivity
Human,Mouse,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas