For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86984A
Western blot detection of SMARCA2/BRM in A549,HL-60,U2OS cell lysates using SMARCA2/BRM Rabbit mAb(1:1000 diluted).Predicted band size:181kDa.Observed band size:190kDa.
Immunohistochemistry of SMARCA2/BRM in paraffin-embedded Human lung cancer tissue using SMARCA2/BRM Rabbit mAb at dilution 1:50
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin.
Immunogen Information
Immunogen
A synthetic peptide of human SMARCA2/BRM
Gene ID
6595
Swissprot
P51531
Synonyms
ATP dependent helicase SMARCA2ATP-dependent helicase SMARCA2BAF190BAF190BBRG1-associated factor 190BBRMFLJ36757Global transcription activator homologous sequencehBRMhSNF2aMGC74511Possible global transcription activator SNF2L2Probable global tr
Calculated MW
48kDa
Observed MW
62kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.