Recombinant SUMO Conjugating Enzyme UBC9 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86987A
Western blot detection of UBE2I/UBC9 in Hela,A549,HL-60 using UBE2I/UBC9 Rabbit mAb(1:1000 diluted)
Immunohistochemistry of UBE2I/UBC9 in paraffin-embedded Human tonsil using UBE2I/UBC9 Rabbit mAb at dilution 1:50
Product Name
Recombinant SUMO Conjugating Enzyme UBC9 Monoclonal Antibody
Catalog Number
RD86987A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
UBE2I is also named as UBC9,UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in thecovalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain,whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues.
Immunogen Information
Immunogen
A synthetic peptide of human UBE2I/UBC9
Gene ID
7329
Swissprot
P63279
Synonyms
Ubiquitin Conjugating Enzyme E2 IRING-Type E3 SUMO Transferase UBC9SUMO-Conjugating Enzyme UBC9Ubiquitin Carrier Protein 9Ubiquitin Carrier Protein IUbiquitin-Protein Ligase ISUMO-Protein LigaseUBC9P18Ubiquitin-Like Protein SUMO-1 Conjugating Enz
Calculated MW
56kDa
Observed MW
56kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.