Recombinant Topoisomerase I Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86962A
Western blot detection of Topoisomerase I in K562,Rat Brain,C6,3T3,Hela cell lysates using Topoisomerase I Rabbit mAb(1:1000 diluted).Predicted band size:91kDa.Observed band size:91kDa.
Immunohistochemistry of Topoisomerase I in paraffin-embedded Human colon cancer tissue using Topoisomerase I Rabbit mAb at dilution 1:50
Immunofluorescence of Topoisomerase I(green) in Hela cells using Topoisomerase I Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant Topoisomerase I Monoclonal Antibody
Catalog Number
RD86962A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Immunogen Information
Immunogen
A synthetic peptide of human Topoisomerase I
Gene ID
7150
Swissprot
P11387
Synonyms
TOP1TOPI
Calculated MW
84kDa
Observed MW
90kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:50-1:100, IF 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.