Recombinant Transcription Factor 7 Like 2 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86960A
Western blot detection of TCF-4 in K562,Hela cell lysates using TCF-4 Rabbit mAb(1:1000 diluted).Predicted band size:68kDa.Observed band size:68kDa.
Immunohistochemistry of TCF-4 in paraffin-embedded Human Cholangiocarcinoma using TCF-4 Rabbit mAb at dilution 1:50
Product Name
Recombinant Transcription Factor 7 Like 2 Monoclonal Antibody
Catalog Number
RD86960A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small inTine.
Immunogen Information
Immunogen
A synthetic peptide of human TCF-4
Gene ID
6934
Swissprot
Q9NQB0
Synonyms
TCF7L2HMG box transcription factor 4HTCF-4T-cell factor-4 variant X2T-cell factor 4Transcription factor 7-like 2TCF-4
Calculated MW
16kDa
Observed MW
16kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.