For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86963A
Western blot detection of Transferrin Receptor in K562,Hela cell lysates using Transferrin Receptor Rabbit mAb(1:1000 diluted).Predicted band size:84kDa.Observed band size:90kDa.
Immunofluorescence of Transferrin Receptor(green) in Hela cells using Transferrin Receptor Rabbit mAb at dilution 1:200, and DAPI(blue)
CD71,also known as transferrin receptor protein 1 (TfR1),is a transmembrane glycoprotein composed of two disulfide-linked monomers,each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Immunogen Information
Immunogen
Recombinant protein of human Transferrin Receptor
Gene ID
7037
Swissprot
P02786
Synonyms
TFRCCD71IMD46T9TFRTFR1TRTRFRp90
Calculated MW
54kDa
Observed MW
50kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:20-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.