Recombinant TRIM25 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86965A
Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.
Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:71kDa.
Immunofluorescence of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody at dilution 1:20, and DAPI(blue)
Immunofluorescence of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody at dilution 1:20, and DAPI(blue)
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
Recombinant TRIM25 Monoclonal Antibody
Catalog Number
RD86965A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Immunogen Information

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Immunogen
A synthetic peptide of human TRIM25
Gene ID
7706
Swissprot
Q14258
Synonyms
E3 ubiquitin/ISG15 ligase TRIM25EFPEstrogen responsive finger proteinEstrogen-responsive finger proteinRING finger protein 147RNF 147RNF147TRI25TRI25TRIM 25Trim25Tripartite motif containing 25Tripartite motif containing protein 25Tripartite m
Calculated MW
151kDa
Observed MW
180kDa

Applications

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Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IF 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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